TAB1 (TGF-beta-activated Kinase 1 and MAP3K7-binding Protein 1, TGF-beta-activated Kinase 1-binding Protein 1, TAK1-binding Protein 1, 3'-Tab1, Mitogen-activated Protein Kinase Kinase Kinase 7-interacting Protein 1, MAP3K7IP1)

TAK1 is a mitogen-activated protein kinase kinase kinase activated by TGF-b and various pro-inflammatory signals (1,2). In vivo TAK1 activation requires its association with TAK1 binding protein 1 (TAB1), which triggers TAK1 autophosphorylation at Thr184 and Thr187 (3,4). The TAB2 adaptor protein links TAK1 with TRAF6 to mediate TAK1 activation following IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK respectively. TAK1 and TRAF6 also activate the NF-kB pathway by phosphorylating the NF-kB inducing kinase (NIK) to trigger subsequent activation of IKK (2,6). In addition to TAK1, TAB1 interacts with and activates p38a (7). Targeted disruption of the TAB1 gene in mice causes a drastic reduction in TAK1 activity and leads to embryonic lethality (8).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T0291-85

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1 Yamaguchi, K. et al. (1995) Science 270, 2008-2011.2 Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.3 Shibuya, H. et al. (1996) Science 272, 1179-1182.4 Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.5 Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.6 Wang, C. et al. (2001) Nature 412, 346-351.7 Ge, B. et al. (2002) Science 295, 1291-1294.8 Komatsu, Y. et al. (2002) Mech. Dev. 119, 239-249.

Additional Info

Detects endogenous levels of total TAB1 protein. Species Crossreactivity: Human and monkey.

Alternative Names

TGF-beta Activated Kinase 1/MAP3K7 Binding Protein 1, MGC57664