TACC2 (Transforming, Acidic Coiled-coil containing protein 2, Anti-Zuai-1, AZU-1, ECTACC)

TACC2, also known as transforming acidic coiled-coil-containing protein 2, belongs to the transforming acidic coiled-coil-containing (TACC) protein family. This group of proteins is defined by its highly conserved coiled-coil domain. This domain is necessary for the assignment of the centrosome-spindle apparatus during cell cycling. There are three proteins belonging to the TACC family; TACC1, TACC2, and TACC3. TACC2 has been reported as playing a necessary role in cultured cell microtubule stabilization. It has also been shown that the binding of TACC2 by T antigen can cause chromosomal instability and mitotic defects (Tei, 2009). Research into the over expression of TACC2 strongly suggests a potential function as a tumor suppressor (Tei, 2009).\n\nApplications:\nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 2ug/ml detected TACC2 on 10ug of mouse testis tissue lysate\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T0297

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Gergely, F., et al., PNAS USA 97: 14352-14357 (2000). 2. Huei-Mei, C., et. al., Molecular Biology 11: 1357-1367 (2000).

Additional Info

Recognizes mouse TACC2, Mr ~65kD. TACC2 has a reported Mr ~73kD in HeLa cell lysates. The immunizing peptide inhibited antibody recognition of the Mr ~65kD band in a competition experiment. Species Crossreactivity: human