TACC3, phosphorylated (Ser558) (Transforming Acidic Coiled-coil Containing Protein 3, ERIC-1, ERIC1)

Transforming acid coiled-coil (TACC) proteins are a family of proteins characterized by a common coiled-coil motif of approximately 200 amino acids at the carboxy-terminal end. Three family members have been identified in humans - TACC1, TACC2 and TACC3. These proteins are thought to be involved in centrosomal microtubule assembly and have been mapped to chromosomal regions that are disrupted in some cancers (reviewed in 2). TACC3 has been shown to be upregulated in many cancer cell lines. When phosphorylated at serine 558 by Aurora A, mammalian TACC3 is localized to mitotic spindles and increases microtubule stability. For this reason, it has been suggested that monitoring the localization of phosphorylated TACC3 would be a useful way to determine the effectiveness of Aurora A inhibitors that show promise as anti-cancer drugs. In addition, studies have shown that TACC3 could be useful as a prognostic marker for non-small cell lung cancer.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T0300-02A

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1.Gergely, F. et al. (2000) Proc Natl Acad Sci U S A 97, 14352-7. 2.Peset, I. and Vernos, I. (2008) Trends Cell Biol 18, 379-88. 3.Still, I.H. et al. (1999) Genomics 58, 165-70. 4.Kinoshita, K. et al. (2005) J Cell Biol 170, 1047-55. 5.Schneider, L. et al. (2007) J Biol Chem 282, 29273-83. 6.LeRoy, P.J. et al. (2007) Cancer Res 67, 5362-70. 7.Tyler, R.K. et al. (2007) Cell Cycle 6, 2846-54. 8.Jung, C.K. et al. (2006) Pathol Int 56, 503-9.

Additional Info

Recognizes endogenous levels of human TACC3 when phosphorylated at Ser558 at ~140kD.