TACE, CT, Blocking Peptide (TNF-alpha Converting Enzyme)

Peptide corresponding to amino acids 807 to 823 of human TACE (1,2). This sequence differs from those of mouse and rat TACE by one amino acid (3).\nTumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-a is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-a. TNF-a converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately (1-3). TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). \n\nTACE also processes other cell surface proteins, including TNF receptor, TGFa, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) (4,5). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association (6).\n\nApplication: \nUsed for blocking the activity of anti-TACE, catalog# T0500. Incubate the peptide with an equal volume of antibody for 30min. at 37 degrees C usually completely blocks the activity of anti-TACE in Western Blotting.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T0500P

Size

50ug

Applications

WB

Form

Supplied as a lyophilized powder.

Purity

Purified 60-70%

References

1. Black RA, Rauch CT, Kozlosky CJ, et al. A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells. Nature 1997;385:729-733\n\n2. Moss ML, Jin SL, Milla ME, et al. Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha. Nature 1997;385:733-736\n\n3. Mizui Y, Yamazaki K, Sagane K, Tanaka I. cDNA cloning of mouse tumor necrosis factor-alpha converting enzyme (TACE) and partial analysis of its promoter. Gene 1999;233:67-74 \n\n4. Peschon JJ, Slack JL, Reddy P, et al. An essential role for ectodomain shedding in mammalian development. Science 1998;282:1281-4\n\n5. Buxbaum JD, Liu KN, Luo Y, et al. Evidence that tumor necrosis factor alpha converting enzyme is involved in regulated alpha-secretase cleavage of the Alzheimer amyloid protein precursor. J Biol Chem 1998;273:27765-7 \n\n6. Giegel DA, Chaplin DD. Targets in cytokine activation. Agents Actions Suppl 1998;49:1-3 \n