TACE, Human, BioAssay(TM) ELISA Kit

Human TACE (TNF-alpha converting enzyme) BioAssay(TM) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TACE. This assay employs an antibody specific for human TACE coated on a 96-well plate. Standards and samples are pipetted into the wells and TACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TACE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. The minimum detectable dose of TACE is typically less than 70pg/ml. \n\nSource:\nHuman\n\nSample Type:\nSerum, plasma, cell culture supernatants and urine.\n\nIntended Use:\nThe BioAssay(TM) kit is used for quantitative protein detection, establishing normal range and validation of antibody array results. The antibody pair provided in this kit recognizes human TACE.\n\nSensitivity:\nThe intra-Assay reproducibility is CV<10% and inter-Assay is CV<12%. \n\nKit Components:\nTACE Microplate (Item A), 1x96wells\nWash Buffer Concentrate, 1x25ml\nStandards (Item C), 2x vials\nAssay Diluent B (Item E) 1x15ml\nDetection Antibody TACE (Item F), 2x vials\nHRP-Streptavidin Concentrate (Item G), 1x200ul\nTMB One-Step Substrate Reagent (Item H), 1x12ml\nStop Solution (Item I), 1x8ml\nAssay Diluent C (Item L), 1x30ml\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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