TAG-72 (Tumor-associated Glycoprotein 72, TAG 72)

Tumor-associated glycoprotein (TAG) 72 is a tumor marker, identified and characterized using two different monoclonal antibodies. It is a mucin-like protein of high molecular weight (220-400KD). The protein is expressed in several epithelial-derived cancers including 94% of colonic adenocarcinomas, 84% of invasive ductal carcinomas of the breast, 96% of non-small cell lung carcinomas, 100% of common epithelial ovarian carcinomas, as well as the majority of pancreatic, gastric, and esophageal cancers evaluated. The protein expression is also detected in fetal colon, stomach, and esophagus, thus defining TAG-72 as an oncofetal antigen. The protein is pancarcinoma in nature. The pancarcinoma distribution and lack of significant reactivity with normal tissues of monoclonal antibody suggests that TAG-72 has thus a potential diagnostic and therapeutic utility for human carcinomas.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry(Paraffin): 1:200-1:400. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to immunostaining. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

138595

Size

20ug

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Thor et al. Cancer Research 46; 3118 1986. 2. Colcher et al. Can Res 48: 4597, 1988. 3. Katari et al. Can Res 50: 4885, 1990. 4. Sheer et al. Can Res 48: 6811, 1988. 5. Filella et al. Anticancer Res 20: 5229, 2000. 6. Negishi et al. Gynecol Oncol. 48:148, 1993.

Additional Info

Recognizes human TAG-72. Stains the majority of human adenocarcinomas including colorectal, pancreatic, gastric, ovarian, endometrial, mammary and non-small cell lung cancer.