Talin, phosphorylated (Ser425) (TLN, Talin 1, Talin-1, TLN1, ILWEQ, KIAA1027)

Focal adhesions connect the cytoskeleton with the extracellular matrix (ECM), a complex structure of secreted macromolecules that surrounds mammalian organs and tissues. Integrins clustered on the extracellular side of focal adhesions signal from the ECM to intracellular protein\ncomplexes, which in turn signal to the actin cytoskeleton to regulate the tension needed for cell motility. Internal signals also converge on focal adhesions to regulate integrin affinity and avidity. Signaling through focal adhesions regulates cell adhesion, migration, proliferation, apoptosis and\ngene expression, and impacts cellular processes such as development, wound healing, immune response, invasion, metastasis and angiogenesis (reviewed in 1-3). Talin is a large, multidomain focal adhesion protein that interacts with the intracellular domains of integrins and other focal\nadhesion proteins. Talin is involved in the formation of focal adhesions and in linking focal adhesions to the actin cytoskeleton (4). The interaction between talin and integrins increases the affinity between integrin and both insoluble and soluble ECM proteins (5,6). Phosphorylation of talin at Ser425 was discovered using mass spectrometry. This site is a potential substrate of CDK5 (7).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T0750-01P

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

(1) Burridge, K. et al. (1988) Annu Rev Cell Biol 4,487-525. (2) Calderwood, D.A. et al. (2000) J Biol Chem 275,22607-10. (3) ffrench-Constant, C. and Colognato, H. (2004) Trends Cell Biol 14, 678-86.\n(4) Nayal, A. et al. (2004) Curr Opin Cell Biol 16, 94-8. (5) Tanentzapf, G. and Brown, N.H. (2006) Nat Cell Biol 8,601-6. (6) Tadokoro, S. et al. (2003) Science 302, 103-6. (7) Ratnikov, B. et al. (2005) J Cell Sci 118, 4921-3.

Additional Info

Recognizes endogenous levels of talin protein only when phosphorylated at Ser425. Species Crossreactivity: monkey, mouse, rat.