Tamm-Horsfall Glycoprotein (Uromucoid, THgp, THP, Uromodulin, UMOD) (BSA, Azide & Glycerol free)

Tamm-Horsfall glycoprotein (THP), which is synthesized by renal tubular cells, is the most abundant protein in normal human urine. Although its physiological function remains unclear, it has been proposed that THP may act as a defense factor against urinary tract infections by inhibiting the binding of S- and P-fimbriated Escherichia coli to renal epithelial cells. Because THP-related proteins are also found in the superficial layers of the oral mucosa, THP may interfere with the cytoadherence of pathogenic bacteria that colonize mucosal surfaces other than those of the urogenital tract. It has been shown that THP binds to virulent Bordetella pertussis and reduces its adherence to both renal and pulmonary epithelial cells. This cytoadherence inhibitory effect was not observed with a B. pertussis mutant lacking the pertussis toxin (PTX) operon, and was dependent on the direct interaction of THP with the S2 subunit within the PTX B oligomer. It has also been shown that the glycosylation moiety of THP is crucial for its binding to PTX. The THP-PTX interaction was exploited to develop an affinity chromatography method that allows a one-step purification of active PTX. These observations suggest that besides its anti-adherence activity, THP may also trap toxins produced by pathogenic bacteria that colonize mucosal surfaces.\nApplications: \nSuitable for use in IEP, RID, Double Diffusion and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nIEP: 100ul antiserum vs. 5ul uromucoid (50mg/L) \nRID and Rocket RID: 10ul antiserum/cm2 in gel vs. 5ul uromucoid (50mg/L), NT-1/5 \nDouble Diffusion: 10ul antiserum vs. 3ul uromucoid (50mg/L)\nNote: The use of 3% PEG 6000, 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH > 8.2) is recommended for the above applications. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T0850B

Size

1ml

Applications

ELISA, IHC

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Glycerol free.

P Type

Pab

Purity

Purified.

Isotype

IgG

References

I. Tamm and F.L Horsfall. Proc. Soc. Exp. Biol. Med. 74, 108-114 (1950) 2. Kidney Int. 16, 279-289 (1979) 3. Science 236, 83-87 (1987) 4. Science 237, 1479-1484 (1987) 5. Microbiology. 2002 Apr;148(Pt 4):1193-201. Interaction of human Tamm-Horsfall glycoprotein with Bordetella pertussis toxin. Menozzi FD, Debrie AS, Tissier JP, Locht C, Pethe K, Raze D.

Additional Info

Recognizes Tamm-Horsfall Glycoprotein. One arc by 2D-IEP against Tamm-Horsfall glycoprotein (1mg/ml). No precipitin line by IEP against normal human plasma. Identity verified by double immunodiffusion.