Tamm-Horsfall Glycoprotein (Uromucoid, THgp, THP, Uromodulin, UMOD) (Lyophilized)

Tamm-Horsfall glycoprotein (Uromucoid, THgp, Uromodulin, UMOD), which is synthesized by renal tubular cells, is the most abundant protein in normal human urine. Although its physiological function remains unclear, it has been proposed that Uromucoid, THgp, Uromodulin, UMOD may act as a defence factor against urinary tract infections by inhibiting the binding of S- and P-fimbriated Escherichia coli to renal epithelial cells. Because Uromucoid, THgp, Uromodulin, UMOD-related proteins are also found in the superficial layers of the oral mucosa, Uromucoid, THgp, Uromodulin, UMOD may interfere with the cytoadherence of pathogenic bacteria that colonize mucosal surfaces other than those of the urogenital tract. It has been shown that Uromucoid, THgp, Uromodulin, UMOD binds to virulent Bordetella pertussis and reduces its adherence to both renal and pulmonary epithelial cells. This cytoadherence inhibitory effect was not observed with a B. pertussis mutant lacking the pertussis toxin (PTX) operon, and was dependent on the direct interaction of Uromucoid, THgp, Uromodulin, UMOD with the S2 subunit within the PTX B oligomer. It has also been shown that the glycosylation moiety of Uromucoid, THgp, Uromodulin, UMOD is crucial for its binding to PTX. The Uromucoid, THgp, Uromodulin, UMOD-PTX interaction was exploited to develop an affinity chromatography method that allows a one-step purification of active PTX. These observations suggest that besides its anti-adherence activity, Uromucoid, THgp, Uromodulin, UMOD may also trap toxins produced by pathogenic bacteria that colonize mucosal surfaces.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: Suitable for use in competitive binding assays and for coating microtitre plates.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C. Reconstitute to nominal volume by adding sterile ddH2O. Aliquot and store at 4 degrees C. Reconstituted product is stable for 12 months at 4 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T0850-05A

Size

1mg

Form

Supplied as a lyophilized powder in 0.02% sodium azide. Reconstitute with sterile dH2O.

Purity

(same/more than) 95%. Purified by SDS-PAGE. Consistent with a molecular weight of approximately 80kD. \nTested negative for and HIV I/II and HCV antibodies and HBsAg.

References

I. Tamm and F.L Horsfall. Proc. Soc. Exp. Biol. Med. 74, 108-114 (1950) 2. Kidney Int. 16, 279-289 (1979) 3. Science 236, 83-87 (1987) 4. Science 237, 1479-1484 (1987) General References: Microbiology. 2002 Apr;148(Pt 4):1193-201. Interaction of human Tamm-Horsfall glycoprotein with Bordetella pertussis toxin. Menozzi FD, Debrie AS, Tissier JP, Locht C, Pethe K, Raze D.