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TANK, Recombinant, Human, aa1-425, His-Tag (TRAF Family Member-associated NFkappa-B Activator Isoform A, I-TRAF, TRAF2)

Cat no: 138753

TANK, Recombinant, Human, aa1-425, His-Tag (TRAF Family Member-associated NFkappa-B Activator Isoform A, I-TRAF, TRAF2)

TANK, also known as TRAF interacting protein, is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby proposing that this protein is an inhibitor of TRAF function that regulates TRAF protein activity by sequestering TRAFs in a latent state in the cytoplasm. Overexpression of TANK inhibits TRAF2-mediated NF-Kappa-B activation signaled by CD40 and both TNF receptors and inhibits LMP1-mediated NF-kappa-B activation by blocking the association of TRAF2 with LMP1. \n\nSource:\nRecombinant protein corresponding to aa1-425 from human TANK at N-terminal, fused to His-tag expressed in E.coli. \n\nMolecular Weight: \n~50.2kD (448aa)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSMDKNIGE QLNKAYEAFR QACMDRDSAV KELQQKTENY EQRIREQQEQ LSLQQTIIDK LKSQLLLVNS TQDNNYGCVP LLEDSETRKN NLTLDQPQDK VISGIAREKL PKVRRQEVSS PRKETSARSL GSPLLHERGN IEKTFWDLKE EFHKICMLAK AQKDHLSKLN IPDTATETQC SVPIQCTDKT DKQEALFKPQ AKDDINRGAP SITSVTPRGL CRDEEDTSFE SLSKFNVKFP PMDNDSTFLH STPERPGILS PATSEAVCQE KFNMEFRDNP GNFVKTEETL FEIQGIDPIA SAIQNLKTTD KTKPSNLVNT CIRTTLDRAA CLPPGDHNAL YVNSFPLLDP SDAPFPSLDS PGKAIRGPQQ PIWKPFPNQD SDSVVLSGTD SELHIPRVCE FCQAVFPPSI TSRGDFLRHL NSHFNGET\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

138753

Size

100ug

Form

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride, 2mM DTT, 50% glycerol.

Purity

~85% (SDS-PAGE)

References

1. Chung J Y., et al. (2002) J Cell Sci. 115:679-688. 2. Rothe M., et al. (1996) Proc Natl Acad Sci USA. 93:8241-8846.

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