TANK (TRAF Family Member-associated NF-kappa-B Activator, TRAF-interacting Protein, ITRAF, I-TRAF, TRAF2)

e range of cell surface receptors, participating in the regulation of cell survival, proliferation, differentiation and stress responses. TRAF-associated NF- B activator (TANK), also known as TRAF-interacting protein (I-TRAF), is a TRAF binding protein that demonstrates both stimulatory and inhibitory properties (5,6). TANK binds to the carboxy domain of the TRAF1, -2 and -3. Overexpression of TANK prevents the association of TRAF2 with TNFR2, inhibiting TNFR2 and CD40 induced NF- B activation (6). TANK is also reported to synergize with low levels of TRAF2 to activate NF- B (5). TANK assists in the activation of NF- B via association and activation of TANK-binding kinase 1 (TBK1) or IKK which promotes activation of the IKK complex (7,8). It has also been shown that TANK may synergize with TRAF2, TRAF5, and TRAF6 but not TRAF3 in SAPK activation (9). TNF stimulation results in IKK -dependent phosphorylation of TANK which may provide negative feedback regulation of TANK mediated NF- B activation (10).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nAA Sequence:\nMDKNIGEQLNKAYEAFRQACMDRDSAVKELQQKTENYEQRIREQQEQLSLQQTIIDKLKSQLLLVNSTQDNNYGCVPLLEDSETRKNNLTLDQPQDKVISGIAREKLPKVDIASAESSI\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

134210

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Cheng, G., et al., (1996), \"TANK, a co-inducer with TRAF2 of TNF- and CD40L-mediated NF-kappaB activation\", Genes Dev., 10: 963-973. 2. Rothe, M., et al., (1996), \"I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction\", Proc. Natl. Acad. Sci. USA, 93: 8241-8246. 3. Pomerantz, J.L., et al., (1999), \"NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase\", EMBO J., 18: 6694-6704. 4. Chariot, A., et al., (2002), \"Association of the adaptor TANK with the I kappaB kinase (IKK) regulator NEMO connects IKK complexes with the IKK epsilon and TBK1 kinases\", J. Biol. Chem., 277: 37029-37036.

Additional Info

Recognizes human TANK.