TAO Kinase 2, phosphorylated (Ser181) (Thousand and One Amino Acid Protein Kinase 2, TAOK2, TAO2, KIAA0881, Kinase from Chicken Homolog C, hKFC-C, MAP3K17, Prostate-derived Sterile 20-like Kinase 1, Prostate-derived STE20-like Kinase 1, PSK1, PSK-1, PSK,

In vitro, TAO (thousand and one amino acid) protein kinase 2 (TAO2) activates MAP/ERK kinases (MEKs) 3, 4, and 6 toward their substrates p38 MAP kinase JNK/SAPK (Chen et al., 1999; Chen and Cobb, 2001). This and more recent work has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs (Chen et al., 2003). Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser181 is located within this domain.\n\nApplications: \nSuitable for use in Western Blot and Dot Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T0650-01

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Xen/Amph

Form

10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg per ml BSA and 50% glycerol

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Chen, Z., Raman, M., Chen, L., Lee, S.F., Gilman, A.G., and Cobb, M.H., "TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors," J. Biol. Chem. 278 (2003) 22,278-22,283.\n2. Chen, Z., and Cobb, M.H., "Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2", J. Biol. Chem. 276 (2001) 16,070-16,075.\n3. Chen, Z., Hutchison, M., and Cobb, M.H., "Isolation of the protein kinase TAO2 and identification of its mitogen-activated protein kinase/extracellular signal-regulated kinase kinase binding domain," J. Biol. Chem. 274 (1999) 28,803-28,807.

Additional Info

Specific for the ~120k TAO2 phosphorylated at Ser181 in Western blots of rat hippocampal lysate. Species Crossreactivity: This antibody recognizes the rat form of the protein. Other species have not been tested. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody.

Alternative Names

EC=2.7.11.1, PSK1-beta