TARP Gamma2-4-8 (Stargazin) (Voltage-dependent calcium channel gamma-2 subunit, Neuronal voltage-gated calcium channel gamma-2 subunit, Stargazin and Stg)

Stargazin belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state. There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.\n\nApplications: \nWestern Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\n1ug/ml was sufficient for detection of TARP Gamma2/4/8 in 20ug of rat brain lysate by Western Blot using Goat anti-mouse IgG:HRP as the secondary antibody.\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167613

Size

100ug

Applications

ICC, WB

Hosts

Mouse

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS pH7.4, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Gill M.B., et al. (2011) J Neurosci. 31(18): 6928-6938.\n2. Yu L. (2011) Neuroscience. 178: 13-20.\n3. Steinmetz C.C., Turrigiano G.G. (2010) J Neurosci. 30: 14685-14690.\n4. Kato. A.S. et al. (2007) J Neurosci. 27: 4969-4977.\n5. Letts V.A., et al. (1998) Nat Genet. 19: 340-34

Additional Info

Recognizes rat TARP Gamma2-4-8 (Stargazin). Species Crossreactivity: Rat, Mouse. Detects ~35-55kD. Does not cross-react with TARPGamma3.