TATA-binding Protein-associated Factor 2N (68 kD TATA-binding Protein-associated Factor, TAF(II)68, TAFII68, RNA-binding Protein 56, RBP56, TAF15, TAF2N)

TAF15 is a member of a family of 3 closely related mammalian RNA binding proteins, the other members being FUS/TLS and EWSR1. TDP43 is a more distant relative of these three. The protein is also known as RBP56, for RNA binding protein 56kD (1). The Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC) name for this protein is TAF15. As with the other members of this family, TAF15 contains one copy of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and DNA. It also has a single Zinc Finger domain of the ZnF_RBZ subtype found in Ran binding proteins. Ran is a small G protein related to p21-Ras which regulates the import and export of proteins to the nucleus. Like TDP43, Fus/TLS and EWS, TAF15 is widely expressed in tissues and is localized primarily in the nucleus of cells (2). It was recently shown that point mutations in TAF15 may be associated with some familial forms of Lou Gehrig's disease, also known as amyotrophic lateral sclerosis or ALS (3). Fus/TLS and TDP43 protein mutations and aggregation have been implicated in various kinds of neurological disease including ALS (4). The HGNC name for this protein is TAF15.\n\nApplications:\nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:500-1:1000\nWestern Blot: 1:1000; A suitable control tissue is HeLa cell lysates.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

031433

Size

100ul

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Morohoshi F, Arai K, Takahashi EI, Tanigami A, Ohki M. Cloning and mapping of a human RBP56 gene encoding a putative RNA binding protein similar to FUS/TLS and EWS proteins. Genomics 1996 38:51-7 (1996).\n\n2. Andersson MK, St

Additional Info

Recognizes TAF15 specifically both in western blots and in immunocytochemical experiments. On blots it eveals a prominent 68 kD band, and on cells in tissue culture the antibody stains mainly in nucleus.