TATA Binding Protein (TBP, a-TBP, GTF2D, GTF2D1, MGC117320, MGC126054, MGC126055, SCA17, TATA Box Binding Protein, TFIID)

The TATA-binding protein (TBP) is believed to function as an essential factor of the general transcription machinery and to be involved in transcription by all three eukaryotic RNA polymerases (pol I, II, and III). TBP specifically binds to TATA element at the promoter region and interacts with numerous transcription factors, including TBP-associated factors (TAFs), activators, and some tumor suppressor proteins. \n\nApplications:\nSuitable for use in Western Blot, Gel Shift and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunoprecipitation: Truncated TBP molecule containing only the N-terminal domain.\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T1024-50

Size

50ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 40% glycerol. No preservatives added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes an epitope within aa1-20 of human TBP. The specific epitope is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA. The epitope is hidden (not recognized) on the full-length protein or when the protein is not complexed. Species Crossreactivity: mouse and rat. Does not crossreact with Drosophila, yeast, silk worm or Xenopus.