Tau, 3-repeat isoform RD3 (Tau Protein, Microtubule-associated Protein, MAP)

Microtubule Associated Proteins, or MAPS, bind to the tubulin subunits of microtubule structures and regulate their functional stability. In the cell MAPs bind to monomer and multimerized tubulin. MAP binding to multimerized tubulin further stabilizes the formation of higher order microtubulin structures. MAP binding to microtubule structures is mediated through phosphorylation through Microtubule Affinity Regulated Kinase (MARK). Phosphorylation releases MAPs bound to microtubules, destabilizing the structure, driving it toward disassembly. There are predominately two MAP types, I, II. Type II MAP includes MAP2, MAP4, and tau and are found in nervous tissue. Six tau isoforms exist in brain tissue, and they are distinguished by their number of binding domains. Three isoforms have three binding domains and the other three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (allowing it to bind to the negatively-charged microtubule). The isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Differential Detection of Tauopathies. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:4000 detects Tau (3-repeat isoform RD3) in lysates from rat brain cytosol fraction. Rat brain cytosol fraction was resolved by electrophoresis, transferred to nitrocellulose and probed with T1030-40B. Proteins were visualized using a IgG (HRP) Gt x Mo and a chemiluminescence detection system.\nImmunohistochemistry: Detects Tau (3-repeat isoform RD3) in autoclaved paraffin brain sections (1). \nDifferential Detection of Tauopathies: See Ref. 2.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\nI1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T1030-40B

Size

200ul

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as a liquid, 0.05% sodium azide. Also available without azide. See T1030-40B1.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. de Silva, R., et al., Neuropathol. Appl. Neurobiol. 29: 288-302 (2003). 2. Togo, T., et al., J. Neuropathol. Exp. Neurol. 61: 547-556 (2002). 3. Hanger, D.P., et al., FEBS Lett. 531: 538-542 (2002).

Additional Info

Recognizes human Tau (3-repeat isoform RD3), Mr 45-65kD. Higher MW bands (68-72kD) represent phosphorylated Tau. Species crossreactivity: rat. Species Sequence homology: mouse and bovine, 100%.