Tau, Phosphorylated (Ser214) (Microtubule-associated Protein Tau, Neurofibrillary Tangle Protein, Paired Helical Filament-tau, PHF-tau, MAPT, MAPTL, MTBT1)

Tau is a collection of microtubule-associated proteins that is involved in microtubule assembly and stabilization. In adult human brain, 6 isoforms, ranging between 352 and 441aa in length, are produced as a result of alternative RNA splicing. The expression of tau isoforms is developmentally regulated, as only the smallest tau polypeptide is expressed in the fetal brain. Hyperphosphorylated Tau is the major component of the paired helical filament of Alzheimer's disease. Anti-phosphor-Tau antibodies are used to identify specific aa that are phosphorylated in Tau from normal brains and Alzheimer's disease brains. The Tau proteins, especially in developing brains and in Alzheimer brains, can be found to be phosphorylated in vivo at many different sites.\n\nApplications:\nSuitable for use in Dot Blot and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nDot Blot: 0.5-2ug/ml\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: T1032-45F1\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T1032-45F

Size

25ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, pH 7.4, 0.05 % sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Cleveland DW, et al (1977) J. Mol. Biol. 116, 207-225. 2. Goedert M, et al (1989) Neuron 3, 519-526. 3. Geodert M, et al (1989) EMBO J. 8, 393-399. 4. Billingsley M et al (1997) Biochem J 323, 577-591.

Additional Info

Recognizes human Tau, when phosphorylated at Serine 214. Species Crossreactivity: mouse, rat and bovine.