Tau, Total, Human (Tau Protein, Microtubule-associated Protein, MAP) BioAssay(TM) ELISA Kit

Tau, Total, Human (Tau Protein, Microtubule-associated Protein, MAP) BioAssay(TM) ELISA Kit is used for the quantitative determination of human total Tau protein. Tau is a microtubule associated protein found predominantly in neuronal axons of vertebrate brain. Tau protein is highly soluble and normally attached to axonal microtubules. Tau stabilizes the microtubules and makes them rigid. Tau interacts with actin in the cytoskeleton and neuronal outgrowth, anchors enzymes such as protein kinases and phosphatases, and regulates intracellular vesicle transport. Tau phosphorylation regulates both normal and pathological functions of this protein. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHFs), the building block of neurofibrillary lesions in AlzheimerпїЅs disease (AD). Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to neuronal degeneration. Deposition of filamentous tau is implicated in other neurodegenerative diseases including cortical basal degeneration (CBD), progressive supranuclear palsy (PSP), PickпїЅs disease and certain forms of ParkinsonпїЅs disease.\n\nSensitivity: \n<12pg/ml\n\nRange: \n31.2-2000pg/ml\n\nIncubation Time: \n4 hours\n\nSample Size: 50ul\n\nAccuracy:\nInter-assay CV: (same/less than)10%\nIntra-assay CV: (same/less than)8%\n\nKit Components:\nT1034-25A: Microtiter Plate 1x96 wells Coated with human Tau antibody\nT1034-25B: Hu Tau (Total) Standard 2x1 vial 3800pg Reconstitute in 1.9ml of T1034-25C\nT1034-25C: Standard Diluent Buffer. 1x25ml \nT1034-25D: Detection Antibody 1x11ml \nT1034-25E: Pab (HRP), 100x 1x125ul\nT1034-25F: HRP Diluent 1x25ml\nT1034-25G: Wash Buffer, 25x 1x100ml\nT1034-25H: TMB 1x25ml \nT1034-25J: Stop Solution 1x25ml\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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