TBK1 (NAK, T2K, TANK-binding kinase 1, NF-KAPPA-B-ACTIVATING KINASE)

Serine/threonine-protein kinase TBK1, also called TANK-binding kinase 1 or NF-kappa-B-activating kinase is an enzyme that in humans is encoded by the TBK1 gene.The gene was assigned to human chromosome 12q14.2. Serine/threonine kinase plays an essential role in regulating inflammatory responses to foreign agents. TBK1 and NF-kappa-B signaling are essential in KRAS mutant tumors, and established a general approach for the rational identification of codependent pathways in cancer.\n\nUniProt Number:\nQ9UHD2\n\nGene ID:\nTBK1\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry (Paraffin).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145250

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Barbie, D. A., Tamayo, P., Boehm, J. S., Kim, S. Y., Moody, S. E., Dunn, I. F., Schinzel, A. C., Sandy, P., Meylan, E., Scholl, C., Frohling, S., Chan, E. M., and 23 others. Systematic RNA interference reveals that oncogenic KRAS-driven cancers require TBK1. Nature 462: 108-112, 2009.\n2. Chien, Y., Kim, S., Bumeister, R., Loo, Y.-M., Kwon, S. W., Johnson, C. L., Balakireva, M. G., Romeo, Y., Kopelovich, L., Gale, M., Jr., Yeaman, C., Camonis, J. H., Zhao, Y., White, M. A. RalB GTPase-mediated activation of the I-kappa-B family kinase TBK1 couples innate immune signaling to tumor cell survival. Cell 127: 157-170, 2006.\n3. Fingert, J. H., Robin, A. L., Stone, J. L., Roos, B. R., Davis, L. K., Scheetz, T. E., Bennett, S. R., Wassink, T. H., Kwon, Y. H., Alward, W. L. M., Mullins, R. F., Sheffield, V. C., Stone, E. M. Copy number variations on chromosome 12q14 in patients with normal tension glaucoma. Hum. Molec. Genet. 20: 2482-2494, 2011.\n4. Fujita, F., Taniguchi, Y., Kato, T., Narita, Y., Furuya, A., Ogawa, T., Sakurai, H., Joh, T., Itoh, M., Delhase, M., Karin, M., Nakanishi, M. Identification of NAP1, a regulatory subunit of I-kappa-B kinase-related kinases that potentiates NF-kappa-B signaling. Molec. Cell. Biol. 23: 7780-7793, 2003.\n

Additional Info

Recognizes human,mouse and rat TBK1. No crossreactivity with other proteins.