TBKBP1 (TANK-binding Kinase 1-binding Protein 1, TBK1-binding Protein 1, TBKBP1, KIAA0775)

TBKBP1 is an adaptor protein belonging to the fibrillar collagen family, consisting of trimers of identical alpha 1 chains which are linked to each other by interchain disulfide bonds. It is essential for signal transduction during viral infection and plays a major role in the NF-kB pathway. TBKBP1 is thus required for efficient IRF (interferon regulatory factor) activation by Sendai virus. The protein binds TBK1 (TANK-binding kinase) and IKKi (inhibitor of nuclear factor B kinase, inducible) via a newly identified TBK1/IKKi-binding domain (TBD), which it shares with TANK and NAP1. Binding to kinases via TBD is essential for IRF3 (interferon regulatory factor 3) activation which controls the expression of antiviral genes during infection. The protein has a ubiquitous expression with highest levels in ovary, followed by testis and brain. Human TBKBP1 gene is mapped to chromosome 17q21.32.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-4.ug/ml\nImmunohistochemistry (formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nBrain\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T0700-91A

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Grigory, R. et al. The EMBO Journal. 26:3180-3190 (2007). 2. Bouwmeester, T. et al. Nature. Cell. Biol. 6:97-105 (2004). 3. Nagase, T. et al. DNA. Res. 5:277-286 (1998).

Additional Info

Recognizes human TBKBP1. Species Crossreactivity: mouse and rat.