TCCR (T Cell Cytokine Receptor, IL-27 R alpha, IL27RA, WSX-1) (PE)

TCCR, also known as WSX-1 and IL-27 Ra, is a 96kD member of the type I group 2 cytokine receptor family.1-4 The extracellular region of human TCCR shares 63% aa identity with the extracellular domain of mouse IL-27 Ra.2, 3 TCCR is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and native T cells.1, 2, 4, 5 As with many other class I cytokine receptor chains, IL-27 Ra heterodimerizes with gp130, the common receptor subunit of IL-6 family cytokines, to form a functional IL-27 receptor.6 IL-27 only binds to IL-27 Ra/WSX-1/TCCR and not the gp130 subunit.7 Studies using WSX-1 mice reveal that WSX-1 has the ability to suppress T cell hyperactivity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity.4, 8, 9 In particular, WSX-1 is believed to induce IL-12 R on native T cells, predisposing them to a Th1 phenotype. This is offset by its ability to limit IL-12 (and TNF) production by macrophages, and its suppression of IFN-g production.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 10ul per (same/less than)1x10e6 cells \nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nNSO myeloma cells from Balb/c mice. \n\nAbsorption: 488nm\n\nEmission: 565-605nm\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

T2009-04B

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 1ml saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG2B

References

1. Villarino, A.V. et al. (2004) J. Immunol. 173:715. 2. Chen, Q. et al. (2000) Nature 407:916. 3. Sprecher, C.A. et al. (1998) Biochem. Biophys. Res. Commun. 246:82. 4. Artis, D. et al. (2004) J. Immunol. 173:5626. 5. Holscher, C. et al. (2005) J. Immunol. 174:3534. 6. Pflanz, S. et al. (2004) J. Immunol. 172:2225. 7. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724. 8. Villarino, A. et al. (2003) J. Immunol. 170:645. 9. Hamano. S. et al. (2003) Immunity 19:657.

Additional Info

Recognizes human TCCR. No cross