TCF-4 (T-cell Transcription Factor 4)

T cell transcription factor-4 (TCF-4) is a member of the TCF-LEF family of transcription factors. TCF-4 interacts with b-catenin in the Wnt signaling pathway to activate transcription of Wnt target genes. TCF-4 plays important roles in embryogenesis and oncogenesis. High levels of TCF-4 expression was found in developing CNS and limb buds. (In adults, TCF-4 is widely expressed.) Brain is the major site of expression, however, TCF-4 mRNA is also found in high levels in liver, lower in heart, lungs, kidneys, and testis, and lowest in spleen and muscle. In human TCF-4, four alternative splice sites are observed which correspond to the synthesis of many hTCF-4 isoforms with short, medium, or long-C-terminal ends (ADDINENRfu). BLAST searches showed these isoforms are very similar to each other, except those that are close to the C-terminus, however, the calculated molecular weights ranged from 52kD to 68kD. For mouse TCF-4, BLAST searches showed different isoforms with calculated molecular weights that range from 29kD to 51kD.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested\n\nRecommended Dilution:\nELISA (Human): 0.1-1ug/ml\nWestern Blot (Rat): 1-5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nRat liver and brain tissue lysates. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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