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TCF Reporter Plasmid Kit, BioAssay(TM)

Cat no: T2029

TCF Reporter Plasmid Kit, BioAssay(TM)

Applications:\nAssessment of Tcf activity in transfected cells using the TOP?ash and the FOP?ash reporter plasmids.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2029

Size

1Kit

References

1. Leung-Hagesteijn, C, et al (2001). Modulation of integrin signal transduction by ILKAP, a protein phosphatase 2C associating with the integrin-linked kinase, ILK1. EMBO J 20: 2160-70. 2. Coghlan, M P, et al (2000). Selective small molecule inhibitors of glycogen synthase kinase-3 modulate glycogen metabolism and gene transcription. Chem Biol 7: 793-803. 3. Staal, F. J., et al (1999). Tcf-1-mediated transcription in T lymphocytes: differential role for glycogen synthase kinase-3 in fibroblasts and T cells. Int Immunol 11: 317-23. 4. Roose, J, et al (1998). The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors. Nature 395: 608-12. 5. van de Wetering, M, et al (1997). Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF. Cell 88: 789-99. 6. Ishitani, T., et al (1999). The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF. Nature 399: 798-802. 7. Korinek, V., et al (1998). Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse. Mol Cell Biol 18: 1248-56.

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