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TCF Reporter Plasmid (TOPflash, TK-luciferase reporter)

Cat no: T2030

TCF Reporter Plasmid (TOPflash, TK-luciferase reporter)

Transfection grade T-cell factor (TCF) reporter plasmid containing two sets (with the second set in the reverse orientation) of three copies of the TCF binding site (wild type) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. FOPflash (F5950) containing mutated TCF binding sites is also available as a negative control.\n\nSize:\n5.5kb\n\nApplications:\nSuitable for use in TCF assays. Other applications not tested.\n\nRecommeded Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T2030

Size

5ug

Form

Supplied as a liquid in 10mM Tris-HCl, pH 8.0, 1mM EDTA.

Purity

Purified by immunoaffinity chromatography.

References

, 1. Coghlan, M.P., et al, Chem. Biol. 10: 793-803, 2000. 2. Ishitani, T., et al., Nature 399: 798-800, 1999. 3. Korinek, V., et al., Mol. Cell. Biol. 18: 1248-1256, 1998.

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