TCF12 (Transcription Factor 12, DNA Binding Protein HTF4, E-box-binding Protein, HEB, HTF4, Transcription Factor HTF4)

TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. TCF12 is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation. Recent reports have shown that TCF12 is also a critical factor required for the development of invariant natural killer T cells.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nImmunocytochemistry: 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146739

Size

100ug

Applications

ELISA, ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Zhang Y and Bina M. The nucelotide sequence of the human transcription factor HTF4a cDNA. DNA Seq.1992; 2:397-403. 2. Murre C, McCaw PS, Vaesin H, et al. Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence. Cell1989; 58:537-44. 3. Wojciechowski J, Lai A, Kondo M, et al. E2A and HEB are required to block thymocyte proliferation prior to pre-TCR expression. J. Immunol.2007; 178:5717-26. 4. D'Cruz LM, Knell J, Fujimoto JK, et al. An essential role for the transcription factor HEB in thymocyte survival, Tcra rearrangement and the development of natural killer T cells. Nat. Immunol.2010; 11:240-9.

Additional Info

Recognizes human TCF12. Species Crossreactivity: mouse and rat.