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TCF3, ID (Transcription Factor E2-alpha, Class B Basic Helix-loop-helix Protein 21, bHLHb21, TCF-3, Transcription Factor ITF-1, Immunoglobulin Enhancer-binding Factor E12/E47, Immunoglobulin Transcription Factor 1, Kappa-E2-binding Factor, Transcription F

Cat no: T2024-01C

TCF3, ID (Transcription Factor E2-alpha, Class B Basic Helix-loop-helix Protein 21, bHLHb21, TCF-3, Transcription Factor ITF-1, Immunoglobulin Enhancer-binding Factor E12/E47, Immunoglobulin Transcription Factor 1, Kappa-E2-binding Factor, Transcription F

TCF-3, also known as E2A and ITF-1, is a member of the E protein homeobox family. TCF-3 regulates checkpoints that allow B lineage commitment and B and T cell receptor gene rearrangement. Two isoforms named E12 and E47 are alternatively spliced within the DNA binding and HLH domain. The region of human TCF-3 used as the immunogen, which is identical for E12 and E47, shares 80% and 79% aa identity with mouse and rat TCF-3, respectively.\n\nApplications:\nSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:128,000\nWestern Blot: 1:128,000, 0.01-0.03ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T2024-01C

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Recognizes human TCF3.

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