TCF3 (Transcription Factor 3, ITF1 (E2A Immunoglobulin Enhancer Binding Factors E12/E47, transcription factor E2-alpha, transcription factor 3, kappa-E2-binding factor, immunoglobulin transcription factor 1, E2A immunoglobulin enhancer-binding factor E12/

Supplier:

United States Biological

Cat no: T2024-01B

TCF3 (Transcription Factor 3, ITF1 (E2A Immunoglobulin Enhancer Binding Factors E12/E47, transcription factor E2-alpha, transcription factor 3, kappa-E2-binding factor, immunoglobulin transcription factor 1, E2A immunoglobulin enhancer-binding factor E12/

The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).[supplied by OMIM]\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:128,000\nWestern Blot: 0.01-0.03ug/ml ~75kD band observed in lysates of cell line Daudi (calculated MW of 67.6kD according to NP_003191.1).\nImmunohistochemistry: 3-5ug/ml In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T2024-01B

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1.Greenbaum S, Lazorchak AS, Zhuang Y. Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes.J Biol Chem. 2004 Oct 22;279(43):45028-35

Additional Info

Recognizes human TCF3.

Alternative Names

transcription factor E2-alpha, transcription factor 3, kappa-E2-binding factor, immunoglobulin transcription factor 1, E2A immunoglobulin enhancer-binding factor E12/E47, MGC129648, MGC129647, E2A, transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47), ITF1, TCF3

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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