TCF3 (Transcription Factor E2-alpha, Class B Basic Helix-loop-helix Protein 21, bHLHb21, TCF-3, Transcription Factor ITF-1, Immunoglobulin Enhancer-binding Factor E12/E47, Immunoglobulin Transcription Factor 1, Kappa-E2-binding Factor, Transcription Facto

The TCF3 gene, also called E2A, encodes two basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. These transcription factors are involved in mediating canonical Wnt signaling, which is very important in a diverse array of cellular functions such as stem cell proliferation, self-renewal, activation, fate determination, differentiation and aging and senescence. They bind beta-catenin and can act as transcriptional activators or repressors for Wnt target genes, and have been shown to regulate specific target genes during CNS development downstream of Wnt signaling. TCF3/Lef complexes are also known to play key roles in controlling cell fate lineages in multipotent skin stem cells.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Brain Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146600

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Korinek V, Barker N, Willert K, et al. Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse. Mol. Cell Biol. 1998; 18:1248-1256. 2. Gribble SL, Kim HS, Bonner J, et al. Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression. Dev. Cell 2009; 136:781-9. 3. Cole MF, Johnstone SE, Newman JJ, et al. Tcf3 is an integral component of the core regulatory circuitry of embryonic stem cells. Genes Dev. 2008;22:746-55. 4. Nguyen H, Rendl M and Fuchs E. Tcf3 governs stem cell features and represses cell fate determination in skin. Cell 2006; 127:171-83.

Additional Info

Recognizes human TCF3. Species Crossreactivity: mouse and rat.