tch homolog 4, Drosophila, INT3)

Notch signaling plays a key role in the normal development of many tissues and cell types, through diverse effects on differentiation, survival, and/or proliferation that are highly dependent on signal strength and cellular context. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. Notch family members share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Multiple human notch proteins (Notch1, Notch2, Notch3 and Notch4) have been identified and they function as a receptors for membrane bound ligands. Notch signaling is also linked to tumorigenesis as first demonstrated by the identification of a recurrent t(7;9)(q34;q34.3) chromosomal translocation involving the human Notch1 gene that is found in a small subset of human pre-T-cell acute lymphoblastic leukemias (T-ALL). Since this discovery, aberrant Notch signaling has been suggested to be involved in a wide variety of human neoplasms. Notch4 functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Notch4 may also be associated with susceptibility to schizophrenia in a small portion of cases.\n\nApplications:\nSuitable for use in ELISA. Other applications have not been tested.\n\nRecommended Dilution:\nELISA: 1:500 by peptide-specific ELISA\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N5375-51

Size

50ug

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% sodium azide, before the addition of glycerol to 40%.\n \n

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Allenspach EJ, Maillard I, Aster JC, Pear WS: Notch signaling in cancer. Cancer Biol. Ther. 1(5): 466-476 (2002).\n2. Leong KG, Hu X, Li L, Noseda M, Larrivee B, Hull C, Hood L, Wong F, Karsan A: Activated Notch4 inhibits angiogenesis: role of beta 1-integrin activation. Mol. Cell. Biol. 22(8): 2830-2841 (2002).\n3. Schnabel M, Fichtel I, Gotzen L, Schlegel J: Differential expression of Notch genes in human osteoblastic cells. Int. J. Mol. Med. 9(3): 229-232 (2002).\n4. Fan JB, Tang JX, Gu NF, Feng GY, Zou FG, Xing YL, Shi JG, Zhao SM, Zhu SM, Ji LP, Sun WW, Zheng YL, Liu WQ, Breen G, St Clair D, He L: A family-based and case-control association study of the NOTCH4 gene and schizophrenia. Mol. Psychiatry 7(1): 100-103 (2002).\n5. Mumm JS, Kopan R: Notch signaling: from the outside in. Dev. Biol. 228(2): 151-165 (2000); review.\n6. Wei J, Hemmings GP: The NOTCH4 locus is associated with susceptibility to schizophrenia. Nat. Genet. 25(4): 376-377 (2000).

Additional Info

Recognizes human Notch 4