TCR, alpha, beta (T Cell Receptor, T-cell Receptor beta-1 Chain C Region, TRBC1, T-cell Receptor alpha Chain C Region, TRAC, TCRA) (Biotin)

The T Cell Receptor alpha/beta (TCR) is expressed on T lymphocytes of all mouse strains tested. Plate-bound or soluble antibody activates a/b TCR-bearing T cells. In vitro and in vivo, the antibody can induce immature thymocytes to undergo apoptosis. In vivo administration of this antibody has been shown to deplete a/b TCR-bearing cells to near completion and prevent graft rejection.\n\nRecommended Secondary Antibody: Streptavidin: R-Phycoerythrin\n\nFusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.\n\nApplications:\nFlow Cytometry: Neat-1:10\nOptimal working dilutions to be determined by researcher.\n\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 lymphocytes in 100ul.

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SPECIFICATIONS

Catalog Number

T2040-02E

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Rat, NHP

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgG1

References

1. Tsuchida, M., Hanawa, H.H., Hirahara, H., Watanabe, H., Matsumoto, Y., Sekikawa, H. and Abo, T. (1994). Identification of CD4- CD8- alpha/beta T cells in the subarachnoid space of rats with experimental autoimmune encephalomyelitis. A possible route

Additional Info

Detects a T cell specific molecule consisting of two disulphide-linked subunits of 40 and 46kD molecular weight, respectively, on 97% of peripheral rat T cells as defined by the OX-52 marker. 3H2910 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the 3H2910 determinant at the same level as peripheral T cells, whereas 94% of CD4- CD8- thymocytes are 3H2910 negative. It is most likely directed at a constant determinant of the rat alpha/beta heterodimeric T cell antigen receptor. Species Crossreactivity: Clone is reported to crossreact with Cynomolgus monkey (4).