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TCR, alpha, beta (T Cell Receptor, T-cell Receptor beta-1 Chain C Region, TRBC1, T-cell Receptor alpha Chain C Region, TRAC, TCRA) (FITC)

Cat no: T2040-02H

TCR, alpha, beta (T Cell Receptor, T-cell Receptor beta-1 Chain C Region, TRBC1, T-cell Receptor alpha Chain C Region, TRAC, TCRA) (FITC)

The T Cell Receptor alpha/beta (TCR) is expressed on T lymphocytes of all mouse strains tested. Plate-bound or soluble antibody activates a/b TCR-bearing T cells. In vitro and in vivo, the antibody can induce immature thymocytes to undergo apoptosis. In vivo administration of this antibody has been shown to deplete a/b TCR-bearing cells to near completion and prevent graft rejection.\n\nFusion Partners: Spleen cells from immunized armenian hamsters were fused with cells of the mouse P3X63Ag.653 myeloma cell line.\n\nRecommended Negative Controls: Hamster IgG Negative Control: FITC\n\nApplications:\nFlow Cytometry: Neat\nOptimal working dilutions to be determined by researcher.\n\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.

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SPECIFICATIONS

Catalog Number

T2040-02H

Size

500ug

Applications

FC

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Kubo, R.T. et al. (1989). Characterization of a monoclonal antibody which detects all murine alpha/beta T cell receptors. J. Immunol. 142: 2736-2742.2. Goodman, T. and LeFrancois, L. (1989). Intraepithelial lymphocytes. J. Exp.

Additional Info

Recognizes the mouse alpha/beta T cell receptor. Does not show any reactivity towards the gamma/delta T cell receptor. This antibody will activate T cells when used in immobilized form (1).

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