TCR, alpha, beta (T Cell Receptor, T-cell Receptor beta-1 Chain C Region, TRBC1, T-cell Receptor alpha Chain C Region, TRAC, TCRA) (Low Endotoxin)

The rat alpha/beta T cell receptor is expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. This clone is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4-CD8-thymocytes are R73 negative.\n\nThis clone is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (2).\n\nApplications:\nSuitable for use in Flow Cytometry, Functional Assays, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul\nImmunohistochemistry (frozen): 1:10-1:100\nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nX63Ag.653 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T2040-02B

Size

500ug

Applications

FC, IHC, IP

Hosts

Mouse

Reactivities

Rat, NHP

Form

Supplied as a liquid in PBS, pH 7.4. No preservative added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Hunig, T. et al. (1989) A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes J. Exp. Med. 169: 73-86. 2. Tsuchida, M. et al. (1994) Identification of CD4- CD8- alpha/beta T cells in the subarachnoid space of rats with experimental autoimmune encephalomyelitis. A possible route by which effector cells invade the lesions. Immunology 81: 420-427. 3. Tomida, S. et al. (1994) Intercellular adhesion molecule-1 and leukocyte function-associated antigen-1 are involved in protection mediated by CD3+TCR alpha beta- T cells at the early stage after infection with Listeria monocytogenes in rats Int. Immunol. 6: 955-961. 4. Matsumoto, Y. et al . (1994) Successful prevention and treatment of autoimmune encephalomyelitis by short term administration of anti-T-cell receptor alpha beta antibody. Immunology. 81: 1-7. 5. Colic, M. et al. (1996) Mechanisms involved in the binding of thymocytes to rat thymic dendritic cells. Dev. Immunol. 5: 37-51. 6. Ariki, S.G. and Roux, M.E. (0) T lymphocyte subpopulations in the intestinal villi of immune deficient rats. In Recent Advances in Mucosal Immunology. Edited by McGhee, J., Mestecky, J., Tlaskalova, H. and Sterzl, J. 7. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim.49: 97-110.

Additional Info

Recognizes a constant determinant on the beta chain of the rat alpha/beta T cell receptor. This antibody is mitogenic for unseparated spleen cells and for purified T cells. Species Crossreactivity: Monkey, Cynomolgus monkey.