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TCR, alpha, beta, V beta 2 (T Cell Receptor) (PE/CY5)

Cat no: T2040-02W4

TCR, alpha, beta, V beta 2 (T Cell Receptor) (PE/CY5)

This antibody precipitates a CD3-associated heterodimer of Mr 88kD (two bands of Mr 48kD and 40kD upon reduction) on chicken peripheral blood T cells.1,2 Deglycosylation of the heterodimer yields two polypeptides of Mr 34kD and 31kD from antibody precipitates. In the chicken, two distinct subpopulations of alpha/beta T cells appear in the thymus subsequent to the appearance of gamma/delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1,2 arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vbeta families, Vb1 and Vb2, respectively.3 Two-color immunofluorescence has revealed that the TCR3+ thymocytes include CD4+CD8-, CD4-CD8+, CD4+CD8+ and CD4-CD8- subpopulations. The TCR3+ thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR3/CD3 complex and most of the cells in this subset are CD4+CD8+. Cells in the other subset express TCR3/CD3 in higher density and are either CD4+CD8- or CD4-CD8+, corresponding to the more mature medullary subset of thymocytes. The TCR3+ cells in the blood and spleen express relatively high levels of the TCR3/CD3 receptor complex and are

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SPECIFICATIONS

Catalog Number

T2040-02W4

Size

100ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Ch/Bird

Form

Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Conjugated to PE/CY5.

P Type

Mab

Purity

Purified

Isotype

IgG1,k

References

1. Chen, C.H., J.T. Sowder, J.M. Lahti, J. Cihak, U. L

Additional Info

Recognizes chicken TCR alpha, beta. Reacts with approximately 9% of thymocytes, 15-25% of blood mononuclear cells and 13% of splenocytes young adult chickens.

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