TCR, Fc Receptor, gamma Subunit (Fc-epsilon RI-gamma, FceRI gamma, High Affinity Immunoglobulin epsilon Receptor gamma-subunit Precursor, IgE Fc Receptor gamma-subunit)

The allergic reaction is mediated through the IgE high affinity receptor (FceRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE FceRI receptor complexes cause the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction. The FceRI receptor is a tetrameric complex, consisting of an a-chain, b-chain and a dimeric g-chain. While the a-chain is primarily involved with the binding of IgE, the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FceRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 0.5-2ug/ml. Detects Fc-epsilon RI-gamma subunit in RIPA lysate of RBL cells. RBL cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with T2040. Proteins were visualized using IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.\nImmunoprecipitation: Reported to immunoprecipitate Fc-epsilon RI-gamma subunit from RBL cells. \nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T2040

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Repetto, B., et al., J. Immunol. 15: 4876-488 (1996). 2. Jouvin, M.H., et al., Sem. Immunol. 7: 29-35 (1995). 3. Maurer, D., et al., J. Immunol. 157: 607-616 (1996). 4. Letourneur, O., et al., J. Immunol. 147: 2652-2656 (1991).

Additional Info

Recognizes and is specific for human Fc-epsilon RI-gamma subunit (~10kD). Species Crossreactivity: rat and mouse.