TCR, gamma, delta (T Cell Receptor) (Biotin)

The TCR gamma, delta heterodimer of the CD3/T-cell receptor complex is clonotypic and consists of Ig-like variable and constant domains (1). It is expressed during thymopoiesis and on the small subpopulation of gamma, delta TCR-expressing T lymphocytes in the periphery. The majority of T cells present in some epithelial tissues are gamma, delta+ and have limited receptor diversity (1,2). The TCR gamma, delta heterodimer recognizes peptide antigen bound to MHC antigens, and subsequent signal transduction mediated by the invariant chains leads to T cell activation (3).\n\nApplications: \nSuitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, In vitro activation of gamma/delta TCR-bearing cells, In vivo depletion of gamma/delta TCR-expressing T cells. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: (same/less than) 0.3ug/10e6 cells.\nImmunohistochemistry: Frozen sections\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2040-06H

Size

500ug

Applications

FC, IHC, IP

Reactivities

Mouse

Conjugates

Biotin

Form

Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with Biotin.

P Type

Mab

Purity

Purified immunoglobulin

Isotype

IgG

References

1. Davis, M.M., Annu. Rev. Biochem. 59: 475 (1990). 2. Allison, J.P. & Havran, W.L., Annu. Rev. Immunol. 9: 679 (1991). 3. Klausner, R.D. & Samuelson, L.E., Cell 64: 875 (1991). 4. Dent, A.L., et al., Nature 343: 714 (1990). 5. Bluestone, J.A., et al., Immunol. Rev. 120: 5 (1991). 6. Houlden, B.A., et al., Cold Spring Harbor Symp. Quant. Biol. 54: 45 (1989). 7. Barrett, T.A., et al., Immunol. Res. 10: 341 (1991).

Additional Info

Recognizes mouse gamma/delta T-cell receptor (TCR) complex. Specific for the gamma, delta heterodimer and plate-bound antibody activates gamma, delta TCR-bearing cells. Does not react with alpha, beta TCR-expressing T cells.