TCR, V beta 16.1 (T Cell Receptor)

The subpopulation of HIS 42 positive T cells contains both CD4 and CD8 positive cells, in the same ratio as found in the peripheral T cell population. When bound to Sepharose beads, HIS 42 induces T cell proliferation in the presence of IL-2. In contrast to lymph node T cells a number of thymocytes are found to express HIS 42 only in the cytoplasm or together with membrane expression. Most bright HIS 42 surface labeled thymocytes are also positive for MRC OX-44, a marker predominantly identifying mature thymocytes. SDS PAGE analysis of the membrane molecules immunoprecipitated by HIS 42 shows a band at 85kD. On reduction it separates to two bands at 35kD and 48kD. From this data it is concluded that HIS 42 is directed against a determinant on the rat T cell receptor, which is common to a small number of T cells. Preliminary data suggests that the determinant is found on the beta chain, associated with the Vb16.1 gene product.\n\nApplications:\nSuitable for use in Immunohistochemistry, Flow cytometry and Immunoprecipitation. Other applications have not been tested. \n\nRecommended Dilutions: \nImmunohistochemistry (frozen): 1:10\nImmunohistochemistry (paraffin): not recommended\nFlow Cytometry: Use 10ul labels 10e6 cells in 100ul.\nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nSpleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T2040-46

Size

2ml

Applications

FC, IHC, IP

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG2b

References

1. Kampinga, J. et al. (1989). A monoclonal antibody determinant of the rat T cell receptor expressed by a minor subset of T-cells. Int. Immunol. 1:289-295.

Additional Info

Recognizes rat TCR, V beta 16.1. Detects a minor subset of T cells.