TCTP, phosphorylated (Ser46) (Translationally controlled tumor protein, TCTP p23 HRF)

Translationally controlled tumor protein (TCTP/p23/HRF) is a ubiquitously expressed and highly conserved protein involved in various cellular processes, including a role as a histamine releasing factor in chronic allergic disease (1). TCTP binds tubulin in a cell cycle dependent manner and is associated with the mitotic spindle (2). In addition, TCTP interacts with the actin cytoskeleton to regulate cell shape (3). In mitosis, TCTP is phosphorylated by Plk at Ser46, decreasing microtubule stability (4,5). TCTP interacts with the small GTPase Rheb, possibly acting as a GEF and thus activating the TORC1 pathway, controlling cell growth and proliferation (6,7). TCTP has also been shown to be involved in apoptosis and cell stress (8-11). In cultured cells, reduction of TCTP expression can cause tumor reversion (12). \n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunohistochemistry (Paraffin): 1:100 using citrate as an unmasking buffer.\nImmunofluorescence (IF-IC): 1:200\nFlow Cytometry: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T2040-38

Size

100ul

Applications

FC, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1. MacDonald, S.M. et al. (1995) Science 269, 688-90, 2. Gachet, Y. et al. (1999) J Cell Sci 112 ( Pt 8), 1257-71, 3. Bazile, F. et al. (2009) Carcinogenesis 30, 555-65, 4. Yarm, F.R. (2002) Mol Cell Biol 22, 6209-21, 5. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7, 6. Dong, X. et al. (2009) J Biol Chem 284, 23754-64, 7. Hsu, Y.C. et al. (2007) Nature 445, 785-8, 8. Susini, L. et al. (2008) Cell Death Differ 15, 1211-20, 9. Bommer, U.A. et al. (2009) Oncogene [Epub ahead of print], 10. Gnanasekar, M. et al. (2009) Biochem Biophys Res Commun 386, 333-7, 11. Gnanasekar, M. et al. (2009) Int J Oncol 34, 1241-6, 12. Tuynder, M. et al. (2002) Proc Natl Acad Sci U S A 99, 14976-81.

Additional Info

Recognizes endogenous levels of human TCTP protein at ~23kD only when phosphorylated at Ser46. In some cell types, may recognizes an 80-100kD protein of unknown origin. \nSpecies Crossreactivity: mouse, rat, monkey