TDRD1 (Tudor Domain-containing Protein 1, Cancer/Testis Antigen 41.1, CT41.1)

DRD is a 120-140kD member of the Tudor family of proteins. It is expressed in spermatocytes and spermatids, and forms part of an RNA-silencing complex that regulates gene expression. TDRD1, through its Tudor and MYND domains, binds methylated PIWI (P-element- induced wimpy testis) protein and PIWI-interacting RNA loading factor, respectively. This serves to promote the recruitment of germline-specific small RNAs into a PIWI ribonucleoprotein complex. Human TDRD1 is 1180aa in length. It contains one MYND-type Zn finger region aa170-206, and four methyl-binding Tudor domains aa312-1048. There are four potential splice variants with predicted molecular weights ranging between 79kD and 133kD. One contains an alternate start site at Met404, another contains an alternative start site at Met22, accompanied by deletions of aa554-610 and 775-831, a third shows a 10aa substitution for 1180aa, while a fourth contains deletions of aa328-375, 554-610 and 1059-1134. Over aa837-968, human TDRD1 shares 66% aa identity with mouse TDRD1.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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