Telomerase (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-associated Protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT)

Telomeres are nucleoprotein structures at the ends of eukaryotic chromosomes. They regulate several crucial cellular functions and participate in the control of complex phenotypes, such as aging and cancer. Cell immortalization is strongly associated with the stabilization of telomere length. Therefore, it is suggested that cancer cells achieve immortalization in part through illegitimate activation of telomerase expression. hTERT is the rate-limiting catalytic subunit of telomerase.\nTelomerase is an enzyme uniquely associated with immortal cell lines and is useful for identifying undifferentiated PSCs.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: 293T whole cell lysates, HeLa whole cell lysates\n\nCellular Localization: Nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2399-02D

Size

100ul

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

As reported

P Type

Mab

Purity

Ascites

Isotype

IgM

References

1. Masutomi K et al. Telomerase maintains telomere structure in normal human cells. Cell. 114(2): 241-53 (2003) [Western Blot, Immunoprecipitation, indirect immunofluorescence, human]\n2. Blasco, M.A., & Hahn, W.C. Evolving views of telomerase and cancer. TRENDS in Cell Biol. Article In Press (2003)\n3. Zhang, J. G., et al. Antigenic Profiling of Glioma Cells to Generate Allogeneic Vaccines or Dendritic Cell-Based Therapeutics. Clin. Cancer Res. 13: 566-575 (2007)

Additional Info

Specific for hTERT. Species Crossreactivity: Human. Other species have not been tested.