Telomerase (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-associated Protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT)

The 3' ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses an RNA component as a template to lengthen telomeres. The telomeric RNA contains a sequence complementary to TTAGGG. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase resulting in the shortening of telomere with each cell division, and ultimately causing chromosomal instability, aging and cell death. Recently, introduction of telomerase into normal human cells has been shown to extend normal cell life by ~20 doublings. Purification of telomerase from the ciliate also revealed two proteins of 43 and 123kD. p123 is a homolog of yeast Est2 (Essential for Telomerase activity). Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132aa; mouse Est2, 1122aa; ~127kD).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.5-1ug/ml. Coat ELISA plates with 1ug/ml control peptide. \nWestern Blot: 1-10ug/ml using ECL. Mouse telomerase detected in testes, thymus and spleen. \nOptimal dilution to be determined by the researcher.\n\nControl Peptide: \nT2399-10: Telomerase, Mouse, Control Peptide \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T2399-15A

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Greenberg, R.A., et al., Oncogene 16: 1723-1730 (1998). 2. Meyerson, M., et al., Cell 90: 785-795 (1997). 3. Nugent, C.I., et al., Genes Develop. 12: 1073-85 (1998). 4. Martin-Rivera, L., et al., PNAS 95: 10,471-10,474 (1998).

Additional Info

Recognizes Telomerase from mouse FM3A at ~125kD. Also reacts with human immortal cell line 293.