Telomerase (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-associated protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT)

The 3' ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses an RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase resulting in shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death. Approximately 4.8 kb of telomeric DNA is lost with each cell division resulting in large number of chromosomal abnormalities. Most recently, introduction of telomerase into normal human cells has been shown to extend normal cell life by ~20 doubling. \nPurification of telomerase from the ciliate also revealed two proteins of 43 and 123kD. p123 is a homolog of yeast Est2 (Essential for Telomerase activity). Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132 aa; mouse Est2, 1122 aa; ~127kD)\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:10,000-50,000\nWestern Blot (ECL): 1:1000-5000 using ECL\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2399-20

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a lyophillized powder, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Greenberg RA at al (1998) Oncogene in press; Meyerson M et al (1997) Cell 90, 785-795; Nugent CI et al (1998) Genes Develop. 12, 1073-85 (review). 2. Martin-Rivera L et al (1998) PNAS 95, 10471-10474;

Additional Info

Recognizes mouse telomerase. No significant sequence homology is seen with yeast telomerase. Species Sequence Homology: rat and human, 100%