Telomerase (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-associated Protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT)

The 3' ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death. Approximately 4.8kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities.\nPurification of telomerase from the ciliate also revealed two protein of 43 and 123kD. p123 is a homolog of yeast Est2 (Essential for Telomerase activity). Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132aa; mouse Est2 1122aa; ~127kD). hEST2 was localized in the nucleus. Yeast telomerase is 988aa protein.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml. Control peptide, #T2399-80, can be used to coat ELISA plates at 1ug/ml. \nWestern Blot: 1-10ug/ml using ECL. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T2399-90

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Yeast/Fun

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Nakamura, T., et al., Science 277: 955-959 (1997). 2. Meyerson, M., et al., Cell 90: 785-795 (1997). 3. Greenberg, R.A., et al., Oncogene 16(13): 1723-1730 (1998). 4. Nugent, C.I., et al., Genes Develop. 12: 1073-1085 (1998).

Additional Info

Recognizes yeast telomerase.