Telomerase, Human (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-associated protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT)

Telomeres are nucleoprotein structures at the ends of eukaryotic chromosomes. They regulate several crucial cellular functions and participate in the control of complex phenotypes, such as aging and cancer. Cell immortalization is strongly associated with the stabilization of telomere length. Therefore, it is suggested that cancer cells achieve immortalization in part through illegitimate activation of telomerase expression. hTERT is the rate-limiting catalytic subunit of telomerase. \n\nApplications:\nSuitable for use in Flow Cytometry/FACS analysis, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: Paraffin\nOptimal dilutions to be determined by the researcher.\n\nRecomme:nded Secondary Antibodies:\nI1905-18D IgM (X-Adsorbed) (FITC) Pab GtxMo\nI1905-04A IgM (X-Adsorbed) (PE) Pab GtxMo\nI1905-28A IgM (X-Adsorbed) (HRP) Pab GtxMo\nI1905-15U IgM (AP) Pab GtxMo\n\nPositive Controls:\n293T whole cell lysates, HeLa whole cell lysates \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T2399-14

Size

100ul

Applications

FC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as liquid, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgM

References

1. Masutomi, K., et al. PNAS. 102(23): 8222-8227 (2005). 2. Jagadeesh, S., et al. Genistein Represses Telomerase Activity via Both Transcriptional Posttranslational Mechanisms in Human Prostate Cancer Cells. 66: 2107-2115 (2006) 3. Masutomi K et al. Cell. 114(2): 241-53 (2003). 4. Zhang Q, Shi S, Liu Y, et al. J Immunol. 183(12):7787-98 (2009).

Additional Info

Recognizes human Telomerase at ~127kD. May see an additional background band at ~110kD.