Telomerase Protein, Mouse, Control Peptide (Tep1, MGC91078, p240, p80 telomerase homolog, Telomerase-associated protein 1, Telomerase protein 1, Telomerase protein component 1, Tp1)

A 19aa Peptide sequence in the middle portion of Mouse TP1. The 19 AA mouse control peptide is 100% homologous with rat and 89% with human TP1\n\nThe 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death. Approx. 4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities.\n\nPurification of telomerase from the ciliate also revealed two protein of 43 and 123kD. p123 is a homolog of yeast Est2 (Essential for Telomerase activity) and show 20% homology. Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned. Est2 is associated with telomerase activity and TP1. Mouse/rat and human TP1 are 2627 and 2629 aa proteins, respectively.\n\nApplications:\nSuitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot due to low MW. Other applications not tested. \n\nRecommended Dilution:\nELISA: 1ug/ml to coat plates.\nAntibody Blocking: 5-10ug peptide.\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2399-65

Size

50ug

Applications

ELISA, WB

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2

Purity

Highly purified

References

1. Meyerson M et al (1997) Cell 90, 785-795; Greenberg RA et al (1998) Oncogene in press; Nugent CI et al (1998) Genes Develop. 12, 1073-85 (review). 2. Harrington L et al (1997) Science 275, 973-977; Nakayama J et al (1997) Cell 88, 875-884.