Telomerase (Tep1, MGC91078, p240, p80 telomerase homolog, Telomerase-associated protein 1, Telomerase protein 1, Telomerase protein component 1, Tp1)

The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death. Approx. 4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml. Using ECL\nELISA: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: \nT2399-65: Telomerase Protein, Mouse, Control Peptide\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T2399-70

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Meyerson M et al (1997) Cell 90, 785-795; 2. Greenberg RA et al (1998) Oncogene in press; 3. Nugent CI et al (1998) Genes Develop. 12, 1073-85 (review). 4. Harrington L et al (1997) Science 275, 973-977; 5. Nakayama J et al (1997) Cell 88, 875-884.

Additional Info

Recognizes mouse telomerase 1. Species sequence homology: rat,100%; human, 89%