Telomerase (TP1, p80 Telomerase Homolog, Telomerase-Associated Protein 1, Telomerase Protein 1, Telomerase Protein Component 1)

Telomerase is an RNA-dependent DNA polymerase that uses an RNA component to add telomeric repeat sequences at the ends of chromosomes (1). Besides the RNA component which serves as the template that specifies the telomeric repeat, the telomerase complex contains a reverse transcriptase protein (TRT) and various accessory proteins including the telomerase-associated protein 1 (TP1) (2,3). Telomerase activity is low in most somatic cells, causing the gradual shortening of telomeres which can ultimately lead to telomere fusion and cell death (4). High levels of telomerase activity are widely seen in cancerous cells and while recent experiments have suggested that telomerase may be a viable target in cancer therapy (5), expression levels of TP1 do not correlate with malignancy (6). At least two isoforms of TP1 are known to exist.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2 ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman kidney tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nSold under license from Geron

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SPECIFICATIONS

Catalog Number

T2399-97

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Greider CW. Telomere length regulation. Annu. Rev. Biochem. 1996; 274:92-7.2. Cong YS, Wright WE, and Shay JW. Microbiol. Mol. Biol. Rev. 2002; 66:407-25.3. Harrington L, McPhail T, Mar V, et al. . Science 1997; 275:973-7.4. Wong JMY and Collins K. Telomere maintenance and disease. Lancet 2003; 362:983-8.5. Cunningham AP, Love WK, Zhang RW, et al. . Curr. Med. Chem. 2006; 13:2875-88. 6. Hu S, Chan HL, Chen MC, et al. J. Formos. Med. Assoc. 2002; 101:593-7.

Additional Info

Recognizes human TP1. Species Crossreactivity: Mouse and rat.