Telomeric Repeat Binding Factor 1 (TRF1, RAP1)

TRF1 is a negative regulator of telomere length maintenance (1). TRF1 has a number of biochemical similarities to Rap1p, a distantly related DNA-binding protein that functions at telomeres in yeast (1,2). Like Rap1p, TRF1 requires two Myb motifs for DNA binding. Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP) (3). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats (3,4). Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Tin2 localizes to telomeres and is essential for proper regulation of telomere length. TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. Recently, it has been shown that TRF1 expression is significantly elevated in patients with acute lymphoblastic leukemia (ALL) compared to those in acute myeloid leukemia (AML) (5). In this study, TRF1 expression tended to be higher in patients without telomere shortening and in those with hTERT expression suggesting that TRF1 may act to monitor telomere length under the condition of up-regulated telomerase activity in some neoplastic cells. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2395-15

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Bianchi A, Smith S, Chong L, Elias P, de Lange T. EMBO J 16 (7) :1785-94 (1997). 2. Broccoli D, Smogorzewska A, Chong L, de Lange T. Nat Genet 17 (2) :231-235 (1997). 3. Smith et al., Science 282:1484-1487, 1998. 4. Smith S, de Lange T. J Cell Sci 112: 3649-56, 1999. 5. Kim S-h, Kaminker P, and Campisi J. Nat. Genet. 23: 405

Additional Info

Recognizes human Telomeric Repeat Binding Factor 1 (TRF1, RAP1)