Telomeric Repeat Binding Factor 2 (TRF2, RAP2)

The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death. Approx. 4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities.\n\nPurification of telomerase from the ciliate also revealed two protein of 43 and 123 kD. p123 is a homolog of yeast Est2 (Essential for Telomerase activity). Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132 aa; mouse Est2 1122 aa; ~127 kD). (1).\n\nTelomeric Repeat binding Factors (TRF1 and TRF2) bind the telomeric TTAGGG repeat. TRFs have been colocalized with telomeric DNA in metaphase cells. TRFs are located at chromosome ends during metaphase. Mouse and human TRF2 are 495 aa and 500 aa, respectively. TRF1 and TRF2 share ~30% homology.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL.\nELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2400-12

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Meyerson M et al (1997) Cell 90, 785-795; Greenberg RA et al (1998) Oncogene in press; Nugent CI et al (1998) Genes Develop. 12, 1073-85 (review).\n2. Chong L et al (1995) Science 270, 1663-1667; Broccoli D et al Gene accession # AF00299 & AF003000.

Additional Info

The 21aa mouse control peptide has no significant sequence homology with TRF1. mouse TRF21 has 73% sequence homology with the human TRF2.