TEM7, ID (Plexin Domain-containing Protein 1, Tumor Endothelial Marker 3, Tumor Endothelial Marker 7, TEM3, PLXDC1)

Tumor endothelial marker 7 (TEM7) was identified through serial analysis of gene expression on endothelial cells isolated from human normal and malignant colorectal tissues. Further experiments verified TEM7 was highly expressed in the endothelium of numerous other cancer types including breast, lung and brain tumors. At least four isoforms of TEM7 are known to exist; these include intracellular, secreted, and membrane-bound forms. A homologous protein, TEM7R (also known as PLXDC2), acts as a binding partner to TEM7 and is also abundantly expressed in the endothelium of malignant colorectal cancer but is absent or rare in normal colon mucosa. High expression of TEM7 is associated with metastasis and poor survival of patients with osteogenic sarcoma.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nImmunofluorescence: 20ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRat Liver Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146211

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. St. Croix B, Rago C, Velculescu V, et al. Genes expressed in human tumor endothelium. Science 2000; 289:1197-202. 2. Nanda A, Buckhaults P, Seaman S, et al. Identification of a binding partner for the endothelial cell surface proteins TEM7 and TEM7R. Cancer Res. 2004; 64:8507-11. 3. Carson-Weber EB, Watkins DN, Nanda A, et al. Cell surface tumor epithelial markers are conserved in mice and humans. Cancer Res. 2001; 61:6649-55. 4. Fuchs B, Mahlum E, Halder C, et al. High expression of tumor endothelial marker 7 is associated with metastasis and poor survival of patients with osteogenic sarcoma. Gene 2007; 399:137-43.

Additional Info

Recognizes human TEM7. Species Crossreactivity: mouse and rat.