TEM8 (Tumor Endothelial Marker 8, Bacillus anthracis (Anthrax) Toxin Receptor, ATR1)

Recently, using SAGE (Serial Analysis of Gene Expression) technology, St. Croix et al, have identified 46 genes, whose expression is specifically elevated in tumor-associated endothelium. Nine of these genes were prominently expressed only in tumor endothelial cells (EC), but were absent or barely detectable in normal ECs, and named as Tumor Endothelial Markers (TEMs, TEM 1-9). Based on TEM8s interaction with the C5 domain of collagen a3(VI), a protein also preferentially expressed in tumor endothelium, it is thought that TEM8 plays an important role in tumor angiogenesis.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml \nExpected size is ~80kD on HUVEC cells and is an 80kD and 85kD double in human colon cell lysates. The size difference is thought to be due to glycosylation as a 70kD band was seen when cell extracts were treated with a glycosidase cocktail.\nImmunohistochemistry (paraffin): 25ug/ml \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nTEM8 transfected cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T2500-05

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Nagase, T., et al., DNA Res. 4; 141-150 (1997). 2. St.Croix, B., et al., Science 289; 1197-1202 (2000). 3. Nanda, A., et al., Cancer Research 64, 817-820 (2004).

Additional Info

Recognizes human TEM8 (Tumor Endothelial Marker 8).